Genomics-guided isolation and identification of active secondary metabolites of <i>Bacillus velezensis</i> BA-26

Here we report the discovery and identification of antibacterial substances from secondary metabolites a new strain Bacillus velezensis BA-26. The whole genome B. BA-26 was sequenced. Its data were annotated, four potentially unknown gene clusters nine known metabolite synthetic analyzed excavated by antiSMASH software. Based on predicted BA-26, material isolated purified extracellular conducted in combination with mass spectrometry data. A total 24 antifungal compounds identified, namely, iturin various fengycins. minimum inhibitory concentration (MIC) to Botrytis cinerea 62.50 µg·mL−1. MICs C14 fengycin A, C16 C18 62.50, 31.25 0.49 µg·mL−1, respectively, indicating that increase number carbon atoms side chain fatty acids improves its biological activity. Two kinds anti-disease fungal fengycin, identified; they found have activities against common pathogenic fungi. Therefore, may be used as control agent. This study enriches information velezensis, elucidates active components substances, provides useful reference for using agent.Supplemental this article is available online at https://doi.org/10.1080/13102818.2021.1934540 .